Vasco and Cruzeiro are set to face off at São Januário on 29 August, with both clubs announcing their first teams ahead of the fixture.
The two sides are keeping close tabs on the Copa do Brasil, bringing greater stakes to the encounter.
Preparations were shown to fans at the stadium before kickoff, as both squads went through their paces.
Managers Pedro Emanuel and Artur Jorge renew a rivalry that began on Portuguese soil, with this fixture marking their first meeting on Brazilian soil.
Broadcast details and betting odds for the game are listed on the usual channels.
The fixture writes a new page in the long history between these two clubs, and both sets of supporters will be watching closely as the managers look to gain the upper hand.
Both sides are pushing for a place in the next round, which makes the outcome all the more significant.